Computational Tools and Resources Supporting CRISPR-Cas Experiments
نویسندگان
چکیده
منابع مشابه
TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery
Precise studies of plant, animal and human genomes enable remarkable opportunities of obtained data application in biotechnology and medicine. However, knowing nucleotide sequences isn't enough for understanding of particular genomic elements functional relationship and their role in phenotype formation and disease pathogenesis. In post-genomic era methods allowing genomic DNA sequences manipul...
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The CRISPR/Cas technology is enabling targeted genome editing in multiple organisms with unprecedented accuracy and specificity by using RNA-guided nucleases. A critical point when planning a CRISPR/Cas experiment is the design of the guide RNA (gRNA), which directs the nuclease and associated machinery to the desired genomic location. This gRNA has to fulfil the requirements of the nuclease an...
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متن کاملThe CRISPR/Cas System
The epigenome is a heritable layer of information not encoded in the DNA sequence of the genome, but in chemical modifications of DNA or histones. These chemical modifications, together with transcription factors, operate as spatiotemporal regulators of genome activity. Dissecting epigenome function requires controlled site-specific alteration of epigenetic information. Such control can be obta...
متن کاملSpecial focus CRISPR-Cas
Six years ago a new sophisticated prokaryotic defense system was identified that was termed CRISPR-Cas (CRISPR, clustered regularly interspaced short palindromic repeats; Cas, CRISPR associated). Parts of it, the mysterious repeat sequences, were already detected in 1987 but their function remained unknown for the next 20 y. The CRISPR-Cas system is an adaptive, heritable defense system that pr...
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ژورنال
عنوان ژورنال: Cells
سال: 2020
ISSN: 2073-4409
DOI: 10.3390/cells9051288